Journal: Critical reviews in clinical laboratory sciences

Article Title: Epigenetic biomarkers: Current strategies and future challenges for their use in the clinical laboratory

doi: 10.1080/10408363.2017.1410520

Figure Lengend Snippet: Epigenetic biomarkers (cf(methylated)DNA, histones, and miRNAs) analyzed from multiple biospecimens (i.e. liquid biopsy, fresh tissue, and FFPE tissue) may allow simultaneous conduction of diagnosis and targeted therapy, therefore, contributing to theragnosis and precision medicine.

Article Snippet: The method is basically the same as those described by Shechter et al. [ 66 ], with the exception of the use of the hypotonic lysis buffer which is not required for serum samples. table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Kit name Manufacturer Biological source Downstream uses Chromatrap® FFPE ChIP Chromatrap FFPE tissue qPCR, sequencing ChIP-IT® FFPE Active Motif FFPE tissue qPCR, sequencing EPIXTRACT® kits Enzo Mammalian cells and tissues, plasma - serum, nuclear proteins Functional studies (WB, PTMs, enzymatic analysis) Histone extraction kit Abcam Mammalian cells and tissues Functional studies (WB, PTMs analysis) EpiQuik total histone extraction kit Epigentek Mammalian cells and tissues Functional studies (WB, PTMs analysis) ChromaFlash chromatin extraction kit Epigentek Cultured cells, fresh, and frozen tissue ChIP, in vitro protein-DNA binding assays and nuclear enzyme assays EZExtractTM core histone isolation kit BioVision Tissues and cultured cells Protein profiling, post-translational modification and epigenetic analyses Open in a separate window Chromatrap® (Porvair Sciences, Norfold, UK); ChIP-IT® (Active Motif.

Techniques: Methylation, Biomarker Discovery, Clinical Proteomics

Journal: Critical reviews in clinical laboratory sciences

Article Title: Epigenetic biomarkers: Current strategies and future challenges for their use in the clinical laboratory

doi: 10.1080/10408363.2017.1410520

Figure Lengend Snippet: Commercial kits and main characteristics of seven kits designed for whole chromatin or core histones isolation and purification from different biological sources.

Article Snippet: The method is basically the same as those described by Shechter et al. [ 66 ], with the exception of the use of the hypotonic lysis buffer which is not required for serum samples. table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Kit name Manufacturer Biological source Downstream uses Chromatrap® FFPE ChIP Chromatrap FFPE tissue qPCR, sequencing ChIP-IT® FFPE Active Motif FFPE tissue qPCR, sequencing EPIXTRACT® kits Enzo Mammalian cells and tissues, plasma - serum, nuclear proteins Functional studies (WB, PTMs, enzymatic analysis) Histone extraction kit Abcam Mammalian cells and tissues Functional studies (WB, PTMs analysis) EpiQuik total histone extraction kit Epigentek Mammalian cells and tissues Functional studies (WB, PTMs analysis) ChromaFlash chromatin extraction kit Epigentek Cultured cells, fresh, and frozen tissue ChIP, in vitro protein-DNA binding assays and nuclear enzyme assays EZExtractTM core histone isolation kit BioVision Tissues and cultured cells Protein profiling, post-translational modification and epigenetic analyses Open in a separate window Chromatrap® (Porvair Sciences, Norfold, UK); ChIP-IT® (Active Motif.

Techniques: Isolation, Purification, Sequencing, Clinical Proteomics, Functional Assay, Extraction, Cell Culture, In Vitro, Binding Assay, Modification

Journal: Critical reviews in clinical laboratory sciences

Article Title: Epigenetic biomarkers: Current strategies and future challenges for their use in the clinical laboratory

doi: 10.1080/10408363.2017.1410520

Figure Lengend Snippet: Proposed miRNAs used as endogenous control in RT-qPCR assays for detecting circulating miRNAs in liquid biopsy.

Article Snippet: The method is basically the same as those described by Shechter et al. [ 66 ], with the exception of the use of the hypotonic lysis buffer which is not required for serum samples. table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Kit name Manufacturer Biological source Downstream uses Chromatrap® FFPE ChIP Chromatrap FFPE tissue qPCR, sequencing ChIP-IT® FFPE Active Motif FFPE tissue qPCR, sequencing EPIXTRACT® kits Enzo Mammalian cells and tissues, plasma - serum, nuclear proteins Functional studies (WB, PTMs, enzymatic analysis) Histone extraction kit Abcam Mammalian cells and tissues Functional studies (WB, PTMs analysis) EpiQuik total histone extraction kit Epigentek Mammalian cells and tissues Functional studies (WB, PTMs analysis) ChromaFlash chromatin extraction kit Epigentek Cultured cells, fresh, and frozen tissue ChIP, in vitro protein-DNA binding assays and nuclear enzyme assays EZExtractTM core histone isolation kit BioVision Tissues and cultured cells Protein profiling, post-translational modification and epigenetic analyses Open in a separate window Chromatrap® (Porvair Sciences, Norfold, UK); ChIP-IT® (Active Motif.

Techniques: Control, Infection, Virus, Clinical Proteomics